Reporter

Part:BBa_K1878000:Design

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


iRFP670


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 81
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 636
    Illegal BsaI.rc site found at 193


Design Notes

The PstI site within the reporter has been removed. There are several BsaI sites within the protein so do not use the BsaI enzyme for Golden Gate DNA Assembly.


Source

iRFP670 is a mutated form of the Rhodopseudomonas palustris bacterial phytochrome photoreceptor.

References

Shcherbakova, D. M. & Verkhusha, V. V. Near-infrared fluorescent proteins for multicolor in vivo imaging. 10, (2013).